Stilling reeling from the events of a zombie apocalypse, a young woman, along with a group of allies, makes her way across a dystopian Japan in search of her mother, the Zombie Queen.

High school student Mizushima receives Valentines Day chocolates from the new student, Monami. Little did she know that the chocolates contained traces of Monami's vampire blood. He gets infected from eating them and Monami confesses that she wants to live with him forever as vampires. Meanwhile, Mizushima decides that he wants to fully become a vampire with Monami's help. Keiko, Mizushima's girl friend, sees the two on the school rooftop kissing and in a state of hysteria, attempts to throw Monami off the roof but falls off herself instead. Keiko dies but her father, Kenji Furano, the mad scientist, resurrects her as Franken girl. Thus begins a deadly combat between Franken Keiko and Vampire Monami in the name of love.

Yuki lives at home in peace until one day a unit of assassins breaks in and kill her mother. In order to revenge herself Yuki becomes a demon of vengeance by donning gothic lolita clothing, using a parasol as a deadly weapon, and executing the guilty in God's name.

Ami's friend, Yoshie, was murdered and desecrated by the Kimura Gang. She was saved and, like Ami in the previous film, received modifications from the same mechanics. Remembering her past, Yoshie decides to avenge herself and Ami.